polyclonal antibodies against lps  (OriGene)

Journal: Frontiers in Microbiology

Article Title: Multi-omics-based investigation of Bifidobacterium’s inhibitory effect on glioma: regulation of tumor and gut microbiota, and MEK/ERK cascade

doi: 10.3389/fmicb.2024.1344284

Figure Lengend Snippet: Bifidobacterium could regulate intra-tumoral microbiota in glioma mice. (A) Slices from tumor tissues of glioma mice were stained with an antibody against bacterial LPS or LTA or PBS to mitigate non-specific staining (×400). (B) Comparison of α-diversity of tumor microbiota, including Chao1, Shannon, and Simpson indices in the Mod and Mod-BIF groups. Mann–Whitney U test. (C) NMDS analysis based on Bray–Curtis distance. (D) Circos of sample and top 5 phylum. A visual circle diagram describing the correspondence between samples and phylum, and the analysis of variance between groups at the phylum level. (E) Circos of sample and top 10 genus, and the analysis of variance between groups at the genus level. (F) Cladogram of LEfSe analysis between the Mod and Mod-BIF groups. (G) Prediction of bacterial metabolite pathway based on PICURSt2 analysis. Mod, Model; BIF, Bifidobacterium . Experimental data are expressed as the mean ± SEM, ** p < 0.01.

Article Snippet: For immunohistochemistry (IHC), the following primary antibodies were used to incubate the slides, respectively, after blocking: bacterial lipopolysaccharide (LPS; HycultBiotech #HM6011, Netherlands), lipoteichoic acid (LTA; Santa Cruz, #sc-57752, United States), occludin (Servicebio #GB111401, China), and zonula occludens 1 (ZO-1; Servicebio #GB111981, China).

Techniques: Staining, Comparison, MANN-WHITNEY

Journal: Pharmaceutics

Article Title: A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

doi: 10.3390/pharmaceutics15010240

Figure Lengend Snippet: Effect of PFP (1, 10, 100 μg/mL) treatment on ( a ) TNF-α, IL-6, or IL-1β release analyzed by ELISA assay in HaCaT cells exposed to LPS-conditioned THP-1 medium (LCTM) for 24 h; ( b ) TNF-α in HaCaT cells stimulated by LPS (200 ng/mL) for 24 h; ( c ) TNF-α in THP-1 cells exposed to LPS 1 µg/mL) for 24h h. Each column represents mean ± SD. Data were analyzed by one-way analysis of variance followed by Tukey:* p < 0.05, *** p < 0.001,**** p < 0.0001 vs. CTRL; ° p < 0.05,°° p < 0.01, °°°° p < 0.001 vs. LCTM or LPS.

Article Snippet: The inflammatory condition was induced by the bacterial lipopolysaccharide (LPS from Gram-negative Salmonella enteritidis , #L7770, Merck KgaA) alone or in association with the oxidative stimulus H 2 O 2 , as reported in each of the following sections.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Pharmaceutics

Article Title: A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

doi: 10.3390/pharmaceutics15010240

Figure Lengend Snippet: Microscope images of H&E staining of co-culture of THP-1 cells and HaCaT cells previously pre-treated with PFP (100 µg/mL) for 2 h and then stimulated with LPS for 24 h. ( a ) Untreated control HaCaT cells, ( b ) HaCaT cells stimulated with LPS, ( c ) HaCaT cells pre-treated with PFP and then stimulated with LPS.

Article Snippet: The inflammatory condition was induced by the bacterial lipopolysaccharide (LPS from Gram-negative Salmonella enteritidis , #L7770, Merck KgaA) alone or in association with the oxidative stimulus H 2 O 2 , as reported in each of the following sections.

Techniques: Microscopy, Staining, Co-Culture Assay, Control

Journal: Pharmaceutics

Article Title: A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

doi: 10.3390/pharmaceutics15010240

Figure Lengend Snippet: Immunofluorescence staining of ( a ) ZO-1 (green) and ( b ) occludin (green) TJs in HaCaT cells unstimulated (CTRL) or PFP-treated (100 µg/mL), or pre-treated with PFP for 2 h and exposed 3 h to LPS+H 2 O 2 . Dapi (blue) stained cell nuclei. Scale bar: 100 µm.

Article Snippet: The inflammatory condition was induced by the bacterial lipopolysaccharide (LPS from Gram-negative Salmonella enteritidis , #L7770, Merck KgaA) alone or in association with the oxidative stimulus H 2 O 2 , as reported in each of the following sections.

Techniques: Immunofluorescence, Staining

Journal: Pharmaceutics

Article Title: A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

doi: 10.3390/pharmaceutics15010240

Figure Lengend Snippet: Western blot analysis of ( a ) filaggrin and ( b ) loricrin protein levels in HaCaT cells unstimulated (CTRL), or PFP-treated (100 µg/mL), or pre-treated with PFP for 2 h and exposed 3 h to LPS+H 2 O 2 . ( c ) Immunoblot represents protein levels of filaggrin and loricrin and the endogenous target tubulin. Each column represents mean ± SD. Data were analyzed by one-way analysis of variance followed by Tukey: **** p < 0.0001 vs. CTRL; ° p < 0.05 vs. LPS+H 2 O 2.

Article Snippet: The inflammatory condition was induced by the bacterial lipopolysaccharide (LPS from Gram-negative Salmonella enteritidis , #L7770, Merck KgaA) alone or in association with the oxidative stimulus H 2 O 2 , as reported in each of the following sections.

Techniques: Western Blot

Journal: Mediators of Inflammation

Article Title: Notch Signaling Pathway Was Involved in Regulating Programmed Cell Death 1 Expression during Sepsis-Induced Immunosuppression

doi: 10.1155/2015/539841

Figure Lengend Snippet: PD-1 and Notch-related molecules levels in LPS-tolerant THP1 cells. THP1 cells were pretreated with 1.0 μ g/mL LPS for 16 h. Then, nontreated and pretreated THP-1 cells were pelleted, washed once in PBS, and resuspended in culture media at 5.0–10.0 × 10 5 cells/mL. Finally, cells of both groups were stimulated with LPS (1.0 μ g/mL) for another 6 hours to make LPS-nontolerant and LPS-tolerant models. The control group was not given any stimulation. After above treatment, cells were washed for three times and total RNA was extracted. The mRNA levels of TNF- α (a), IL10 (b), PD-1 (c), Jagged1 (d), and Hes1 (e) and protein levels of PD-1 (f) were examined in control, LPS-tolerant, and LPS-nontolerant groups. Data shown are normalized to β -actin mRNA and presented as 2 −ΔΔCT . # P value < 0.05.

Article Snippet: THP1 cells were pretreated with 1.0 μ g/mL Gram-negative LPS ( Escherichia coli 0111:B4; Sigma-Aldrich, St. Louis, MO, USA) for 16 h. Then, nontreated and pretreated THP-1 cells were pelleted, washed once in PBS, and resuspended in culture media at 5.0–10.0 × 10 5 cells/mL.

Techniques:

Journal: Mediators of Inflammation

Article Title: Notch Signaling Pathway Was Involved in Regulating Programmed Cell Death 1 Expression during Sepsis-Induced Immunosuppression

doi: 10.1155/2015/539841

Figure Lengend Snippet: Notch inhibition by DAPT decreased PD-1 and IL-10 transcriptions in LPS-tolerant THP1 cells. LPS-tolerant THP1 cells were induced as mentioned above. In tolerant group, DAPT (40 μ M) was added into the culture at the beginning of the treatment of LPS to inhibit the Notch signaling pathway. The mRNA levels of PD-1 (a), IL-10 (c), and TNF- α (d) and protein levels of PD-1 (b) were examined in control, LPS-nontolerant, LPS-tolerant, and DAPT groups. Data are normalized to β -actin mRNA and presented as 2 −ΔΔCT . # P value < 0.05.

Article Snippet: THP1 cells were pretreated with 1.0 μ g/mL Gram-negative LPS ( Escherichia coli 0111:B4; Sigma-Aldrich, St. Louis, MO, USA) for 16 h. Then, nontreated and pretreated THP-1 cells were pelleted, washed once in PBS, and resuspended in culture media at 5.0–10.0 × 10 5 cells/mL.

Techniques: Inhibition